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Original Research

Open Access

Evaluation of E6 and E7 mRNA expression in HPV DNA positive breast cancer

  • A. Frega1
  • L. Lorenzon2
  • M. Bononi3
  • A. De Cesare3
  • A. Ciardi4
  • D. Lombardi1
  • C. Assorgi1,*,
  • M. Gentile1
  • M. Moscarini1
  • M.R. Torrisi5
  • D. French5

1Department of Woman’s Health and Territorial Medicine, Sant’Andrea Hospital, Faculty of Medicine and Psychology, Rome, Italy

2Surgical and Medical Department of Clinical Sciences, Biomedical Technologies and Translational Medicine, Sant’Andrea Hospital, Faculty of Medicine and Psychology, Rome, Italy

3Department of Surgery “Pietro Valdoni”, Universitary Hospital, Faculty of Medicine and Odontoiatry, Rome, Italy

4Department of Experimental Medicine, Universitary Hospital, Faculty of Medicine and Odontoiatry, Rome, Italy

5Department of Clinical and Molecular Medicine, Sant’Andrea Hospital, Faculty of Medicine and Psychology University of Rome “La Sapienza”, Rome, Italy

DOI: 10.12892/ejgo201202164 Vol.33,Issue 2,March 2012 pp.164-167

Published: 10 March 2012

*Corresponding Author(s): C. Assorgi E-mail: doctoress@hotmail.it

Abstract

Several studies have suggested a possible role for HPV in the pathogenesis of the breast cancer. We investigated the presence of the HPV DNA in breast cancers and non malignant disease breast tissues by the use of a standard HPV detection method (INNO-Lipa HPV), in order to detect HPV DNA in metastatic nodes, to investigate a possible cervical HPV co-infection, and to evaluate the E6/E7 mRNA expression in HPV DNA positive breast cancer tissues. The rate of HPV infection was significantly higher in the cancer group than in controls (9/31 vs 0/12, p = 0.04). One out of eight metastatic axillary nodes was positive for HPV infection; 2/3 of the positive HPV breast cancer patients were co-infected at the cervical site. The role of the virus in breast oncogenesis is still unclear, since our analysis failed in demonstrating the expression of viral E6 and E7 in positive HPV positive breast tumor tissues.

Keywords

Breast cancer; E6; E7; HPV; HPV 16

Cite and Share

A. Frega,L. Lorenzon,M. Bononi,A. De Cesare,A. Ciardi,D. Lombardi,C. Assorgi,M. Gentile,M. Moscarini,M.R. Torrisi,D. French. Evaluation of E6 and E7 mRNA expression in HPV DNA positive breast cancer. European Journal of Gynaecological Oncology. 2012. 33(2);164-167.

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