Establishment and characterization of two cell lines (HEC-155, HEC-180) derived from uterine papillary serous adenocarcinoma

Uterine papillary serous adenocarcinoma (UPSC) is an uncommon histologic subtype of endometrial cancer that characteristically behaves aggressively with a poor prognosis. We established two novel cell lines derived from UPSC designated HEC-155 and HEC-180. Both cell lines have been growing steadily in monolayer cultures for over ten years. Overexpression of p53, Ki67 and p27 was detected in both cell lines by immunohistochemistry. Using a DNA sequencing technique, a point mutation of p53 was detected in exon 8, codon 286 in HEC-155 and in exon 6, codon 195 in HEC-180. These newly established cell lines should be useful for investigating the characteristics of UPSC.

Uterine papillary serous carcinoma (UPSC); Cell line; p53 mutation

[1] Jeffrey J.F., Krepart G.V., Lotocki R.J.: "Papillary serous adenocarcinoma of the endometrium". Obstet. Gynecol., 1986, 67, 670.

[2] Ward B.G., Wright R.G., Free K.: "Papillary cacinomas of the endometrium". Gynecol. Oncol., 1990, 39, 347.

[3] Gitsch G., Friedlander M.L., Wain G.V., Hacker N.F.: "Uterine papillary serous carcinoma". Cancer, 1995, 75 (9), 2239.

[4] Kuramoto H., Hamano M., Imai M.: "HEC-1 cells". Human Cell., 2002, 15 (2), 81.

[5] Seabright M.: "A rapid banding technique for human chromosomes". Lancet, 1971, 2, 971.

[6] Orita M., Suzuki Y., Hayashi K.: "Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction". Genomics, 1989, 5, 874.

[7] GoffB.A., Kato D., Schmidt RA., Ek M., Ferry J.A., Muntz H.G., et al.: "Uterine papillary serous carcinoma : patterns of metastatic spread". Gynecol. Oncol., 1994, 54, 264.

[8] Nordstrom B., Strang P., Lindgren A., Bergstrom R., Tribukait B.: "Endometrial carcinoma: the prognostic impact of papillary serous carcinoma (UPSC) in relation to nuclear grade, DNA ploidy and p53 expression". Anticancer Res., 1996, 16, 899.

[9] Zheng W., Cao P., Zheng M., Kramer E.E., Godwin T.A.: "p53 overexprssion and bcl-2 persistence in endometrial carcinoma : comparison of papillary serous and endometrioid subtypes". Gynecol. Oneal., 1996, 61, 167.

[10] Tashiro H., Isacson C., Levine R., Kurman R.J., Cho K.R., Hedrick L.: ·"·po:,5J3 ge ene mutat10ns are common m utenne serous carcinoma and occur early in their pathogenesis". Am. J. Pathol., 1997, 150 (1), 177.

[11] Hockenbery D.M., Nunez G., Milliman C., Schreiber R.D., Korsmeyer S.J.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death". Nature, 1990, 348, 334.

[12] Ohkouchi T., Sakuragi N., Watari H., Nomura E., Todo Y., Yamada H., Fujimoto S.: "Prognostic significance of Bcl-2, p53 overexpression, and lymphnode metastasis in surgically staged endometrial carcinoma". Am. J. Obstet. Gynecol., 2002, 187 (2), 353.

[13] Polyak K., Kato J.Y., Solomon M.J., Sherr C.J., Massague J., Roberts J.M., Koff A.: "p27Kip l , a cyclyn-Cdk inhibitor, links transforming growth factor-B and contact inhibition to cell cycle arrest". Gene. Dev., 1994, 8, 9.

[14] Shiozawa T., Nikaido T., Nakayama K., Lu X., Fujii S.: "Involvement of cyclin-dependent kinase inhibitor p27Kipl in growth inhibition of endometrium in the secretory phase and of hyperplastic endometrium treated with progesterone". Mo/. Human Reprod., 1998, 4 (9), 899.

[15] Bamberger A.M., Riethdorf L., Milde-Langosch K., Bamberger C.M., Thuneke I., Erdmann I. et al.: "Strongly reduced expression of the cell cycle inhibitor p27 in endometrial neoplasia". Virchows Arch., 1999, 434, 423.

[16] Nycum L.R., Smith L.M., Farley J.H., Kost E.R., Method M.W., Birrer M.J.: "The role of p27 in endometrial carcinoma". Gynecol. Oneal., 2001, 81 (2), 242.

[17] Watanabe J., Sato H., Kanai T., Karnata Y., Jobo T., Hata H. et al.: "Paradoxical expression of cell cycle inhibitor p27 in endometrioid adenocarcinoma of the uterine corpus - correlation with proliferation and clinicopathological parameters". Br. J. Cancer, 2002, 81 (1), 81.

[18] Schmitz M.J., Hendricks D.T., Farley J., Taylor R.R., Geradts J., Rose G.S., Birrer M.J.: "p27 and cyclin Dl abnormalities in uterine papillary serous carcinoma". Gynecol. Oneal., 2000, 77, 439.